Statistical and Media Engineering Approaches to Enhance the Butanol Production From Isolated Microbial Strains

Chandrasekharan Nair Salini; Nair M Lakshmi; Mohan Binoop; Raveendran Sindhu; Ashok Pandey; Parameswaran Binod

Authors

Chandrasekharan Nair Salini Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary, Science and Technology (CSIR-NIIST), Thiruvananthapuram 695 019, Kerala, India | Academy of Scientific and Innovative Research (AcSIR), Thiruvananthapuram 695 019, Kerala, India
Nair M Lakshmi Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary, Science and Technology (CSIR-NIIST), Thiruvananthapuram 695 019, Kerala, India | Academy of Scientific and Innovative Research (AcSIR), Thiruvananthapuram 695 019, Kerala, India
Mohan Binoop Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary, Science and Technology (CSIR-NIIST), Thiruvananthapuram 695 019, Kerala, India
Raveendran Sindhu Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary, Science and Technology (CSIR-NIIST), Thiruvananthapuram 695 019, Kerala, India
Ashok Pandey Centre for Innovation and Translational Research CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31MG Marg, Lucknow-226 001, India
Parameswaran Binod Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary, Science and Technology (CSIR-NIIST), Thiruvananthapuram 695 019, Kerala, India | Academy of Scientific and Innovative Research (AcSIR), Thiruvananthapuram 695 019, Kerala, India

Keywords:

Butanol, Fermentation, Biofuels, Media Engineering, Clostridium sporogenes

Abstract

Anaerobic butanol producing bacteria were isolated, among them Clostridium sporogens found to be a potent producer of butanol with the production of 0.1 g/L of butanol. Individual fermentation parameters including inoculum age (10 - 24 hrs), size (1 % - 9 % v/v) were optimized and maximum production was observed on 12 hrs old 8 % (v/v) inoculum. From the single parameter optimization results, for further improvement in the production of butanol, media engineering was done using Plackett–Burman (PB) and Central Composite Design (CCD). Production of butanol increased to 1.5 g/L and the effect Keywords: of different media components in butanol production is evaluated.

Author Biography

Chandrasekharan Nair Salini, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary, Science and Technology (CSIR-NIIST), Thiruvananthapuram 695 019, Kerala, India | Academy of Scientific and Innovative Research (AcSIR), Thiruvananthapuram 695 019, Kerala, India

Anaerobic butanol producing bacteria were isolated, among them Clostridium sporogens found to be a potent producer of butanol with the production of 0.1 g/L of butanol. Individual fermentation parameters including inoculum age (10 - 24 hrs), size (1 % - 9 % v/v) were optimized and maximum production was observed on 12 hrs old 8 % (v/v) inoculum. From the single parameter optimization results, for further improvement in the production of butanol, media engineering was done using Plackett–Burman (PB) and Central Composite Design (CCD). Production of butanol increased to 1.5 g/L and the effect Keywords: of different media components in butanol production is evaluated.