Utilization of municipal solid waste for bioethanol production using Trichoderma reesei and Saccharomyces cerevisiae

Authors

  • G. Baskar Department of Biotechnology, St. Joseph’s College of Engineering, Chennai, India
  • S. Jose Pandian Department of Biotechnology, St. Joseph’s College of Engineering, Chennai, India
  • S. My. Raghul Reddy Department of Biotechnology, St. Joseph’s College of Engineering, Chennai, India
  • K.S.T. Ramya Department of Biotechnology, St. Joseph’s College of Engineering, Chennai, India
  • R. Naveenkumar Department of Biotechnology, St. Joseph’s College of Engineering, Chennai, India | Biological Systems Engineering, University of Wisconsin–Madison, Madison, WI 53706, United States America | USDA Forest Service Forest Products Laboratory, Madison, WI 53726, United States America
  • R. Pravin Department of Biotechnology, St. Joseph’s College of Engineering, Chennai, India

Keywords:

Municipal solid waste, bioethanol, Optimization, Trichoderma reesei, Saccharomyces cerevisiae

Abstract

In the present study, the utilization of municipal solid waste such as banana peel, paper waste, corn and rice waste was examined for the production of bioethanol. The pre-treatment of the solid waste was done by using different acids such as HNO3, CH3COOH, H2SO4, HCl with different concentrations and also using ultrasonication method. The conditions of using H2SO4 (2%), ultrasonication power (80), temperature (60!) and time (30 min) shows better yields. Trichoderma reesei was used for the saccharification of the substrate and reducing sugar yield of 115.5 mg/ml was achieved using the optimized conditions of 40% of biomass concentration, pH of 5, temperature and time of 36! for 96 hrs. The bioethanol production was done through fermentation using saccharomyces cerevisiae and highest yield of bioethanol with 21.43 mg/g was achieved. The optimized conditions were concluded from the results of using 4% of the yeast concentration with the temperature and time of 32! for 96 hrs. Thus, the results show that municipal solid waste can be utilize defficiently for the production of bioethanol.

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Published

29-03-2023

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Articles