Development of a Two-stage Impaction Based Bioaerosol-cum-particulate Matter Sampler and its Use for Ambient Aerosol Profile

Abstract

Air contains a mixture of various biological and non-biological components. During breathing, human body does not segregate between these components. Studies have provided sufficient proof about the deteriorating effects of degrading air quality on human health. The deterioration in ambient air quality and the emergence of new pollutants in developing countries is also giving rise to the requirement of simple, customized and economical air sampling devices. Relatively simple design, and low cost as compared to real time optical instruments have led to the development of various kinds of impactors. The present study presents a low volume (Q = 12 LPM), two stage (PM10 and PM0.6), impaction based device that can be used for bioaerosol and particulate matter sampling. The device is fabricated of brass and chrome plating is done over it. This device eliminates the need for two different sampling devices to be bought for bioaerosol and particulate matter sampling separately, which is of huge economical advantage for developing nations. Developed device is tested in laboratory conditions and field conditions as well. Laboratory testing was carried out for parametric characterization and optimization of the device. Yearlong sampling for PM0.6 and bioaerosols as Gram positive bacteria, Gram negative bacteria and fungi was carried out within the IIT Kanpur campus and the results were observed in reference to similar studies in the region.For the first time, yearlong bioaerosol inventory was generated for the IIT Kanpur campus.