Biodegradation of Hexavalent Chromium by Acclimatized Pseudomonas Putida: Optimization and Kinetic Study

Abstract

Hexavalent Chromium (Cr (VI)) is considered as priority pollutants to human health and aquatic life. The USEPA has set the maximum permissible limit of 0.05 mg/L of Cr (VI) in domestic uses of water. In the present work, the acclimatized culture of Pseudomonas putida (MTCC 1072) was used for the degradation Cr (VI) in wastewater. The process variables such as pH, temperature, and process time were optimized and found to be 7.0±0.2, 35±2 °C, and 8.0 days, respectively in the batch reactor. The optimized process condition was used to scale-up the batch process at lobortory scale. The maximum elimination of 86.4% was obtained at the optimum condition. The kinetics of biodegradation were studied using Monod and Andrew-Haldane model.