Response Surface Modeling and Optimization of Culture Media in Fermentative Production of 2,3-butanediol

Sulfath Hakkim Hazeena; Parameswaran Binod; Ashok Pandey

Authors

Sulfath Hakkim Hazeena Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, India | Centre for Innovation and Translational Research, CSIR-Indian Institute of Toxicology Research, Lucknow-226001, India
Parameswaran Binod Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, India
Ashok Pandey Centre for Innovation and Translational Research, CSIR-Indian Institute of Toxicology Research, Lucknow-226001, India

Keywords:

2,3-Butanediol, Enterobacter cloacae, Statistical optimization, Response surface methodology

Abstract

2,3-Butanediol is a promising platform chemical with extensive industrial applications.The present study aims to develop a microbial fermentation approach for the production of 2,3-Butanediol. A wild strain of bacteria, Enterobacter cloacae SG1,was studied its capacity to ferment glucose to 2,3- butanediol. Batch fermentation parameters were optimized and media engineering was performed using statistical approach by response surface methodology for increasing the yield of 2,3-Butanediol. At the optimized condition, 25.86 g/L 2,3-butanediol could be obtained from Box-Behnken design with 0.36g BDO/g of glucose. The optimization resulted in almost two times increase in the production of 2,3-Butanediol.